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Rabbit Anti-REC8  antibody (bs-7723R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7723R
英文名称 REC8
中文名称 减数分裂重组蛋白家族REC8抗体
别    名 Cohesin Rec8p; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog (yeast); REC8_HUMAN; REC8L; Rec8L1; Rec8p.  
Specific References  (2)     |     bs-7723R has been referenced in 2 publications.
[IF=6.831] Shangren Wang. et al. Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction. 2022 Mar 17  WB ;  Rat.  
[IF=1.635] Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2018)J Reprod Dev.  WB ;  Mouse.  
研究领域 细胞生物  细胞周期蛋白  细胞分化  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Pig,Horse,Rabbit,GuineaPig)
产品应用 WB=1:500-2000, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 60kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human REC8: 22-120/547 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Function:
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).

Subunit:
Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51.

Subcellular Location:
Nucleus. Chromosome.

Tissue Specificity:
Expressed in testis and thymus.

Similarity:
Belongs to the rad21 family.

SWISS:
O95072

Gene ID:
9985

Database links:

Entrez Gene: 9985 Human

Entrez Gene: 56739 Mouse

Entrez Gene: 290227 Rat

Omim: 608193 Human

SwissProt: O95072 Human

SwissProt: Q8C5S7 Mouse

SwissProt: Q6AYJ4 Rat

Unigene: 419259 Human

Unigene: 23149 Mouse

Unigene: 108612 Rat




产品图片
Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8  (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-REC8 antibody (bs-7723R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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