| 产品编号 | bs-7723R |
| 英文名称 | Rabbit Anti-REC8 antibody |
| 中文名称 | 减数分裂重组蛋白家族REC8抗体 |
| 别 名 | Cohesin Rec8p; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog(yeast); REC8_HUMAN; REC8L; Rec8L1; Rec8p. |
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Specific References (2) | bs-7723R has been referenced in 2 publications.
[IF=6.831] Shangren Wang. et al. Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction. 2022 Mar 17 WB ; Rat.
[IF=1.635] Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2018)J Reprod Dev. WB ; Mouse.
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| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human REC8: 22-120/547 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Function: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity). Subunit: Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51. Subcellular Location: Nucleus. Chromosome. Tissue Specificity: Expressed in testis and thymus. Similarity: Belongs to the rad21 family. SWISS: O95072 Gene ID: 9985 Database links: Entrez Gene: 9985 Human Entrez Gene: 56739 Mouse Omim: 608193 Human SwissProt: O95072 Human SwissProt: Q8C5S7 Mouse Unigene: 419259 Human Unigene: 23149 Mouse Unigene: 108612 Rat
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| 产品图片 |
Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-REC8 antibody (bs-7723R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
