[IF=8.44] Wang Wei. et al. Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis. J TRANSL MED. 2023 Dec;21(1):1-18  IHC,WB ;  Human,Mouse.  

PubMed:37248471

Sample: Lane 1: Mouse Thymus tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human A431 cell lysates Lane 4: Human MCF-7 cell lysates Lane 5: Human HeLa cell lysates Lane 6: Human U251 cell lysates Lane 7: Human Jurkat cell lysates Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kDa Observed band size: 131 kDa

Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-CAPG2 (bs-7721R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131 kD

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CAPG2) Polyclonal Antibody, Unconjugated (bs-7721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (bs-7721R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.