产品编号 | bs-6893R |
英文名称 | KLLN |
中文名称 | Killin-p53调节复制抑制蛋白抗体 |
别 名 | KILIN_HUMAN; Killin; killin, p53-regulated DNA replication inhibitor; KLLN. |
研究领域 | 细胞生物 染色质和核信号 细胞凋亡 细胞周期蛋白 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human |
产品应用 | Flow-Cyt=1ug/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 20kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human KLLN: 20-120/178 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA. Function: DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA. Subcellular Location: Nucleus. SWISS: B2CW77 Gene ID: 100144748 Database links: Entrez Gene: 100144748 Human Omim: 612105 Human SwissProt: B2CW77 Human Unigene: 559820 Human |
产品图片 |
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-KLLN antibody (bs-6893R) Dilution:1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |