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Rabbit Anti-STK18  antibody (bs-6393R)  
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-6393R
英文名称 Rabbit Anti-STK18  antibody
中文名称 丝氨酸/苏氨酸蛋白激酶18抗体
别    名 PLK 4; PLK-4; PLK4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18.  
研究领域 细胞生物  免疫学  信号转导  细胞周期蛋白  激酶和磷酸酶  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse)
产品应用 WB=1:500-2000,Flow-Cyt=2ug/Test,ICC/IF=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 108kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PLK4/STK18: 31-130/970 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.

Function:
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.

Subunit:
Homodimer. Interacts with CEP152 (via N-terminus).

Subcellular Location:
Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus, nucleolus.

Post-translational modifications:
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

SWISS:
O00444

Gene ID:
10733

Database links:

Entrez Gene: 10733 Human

Entrez Gene: 20873 Mouse

Entrez Gene: 310344 Rat

SwissProt: O00444 Human

SwissProt: Q64702 Mouse

SwissProt: B2GUY1 Rat

Unigene: 172052 Human

Unigene: 3794 Mouse

Unigene: 14848 Rat



产品图片
Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-STK18 (bs-6393R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kDa Observed band size: 110 kDa
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (STK18) polyclonal Antibody, Unconjugated (bs-6393R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control(black line):MCF7. Primary Antibody (green line): Rabbit Anti-STK18 antibody (bs-6393R) Dilution:2ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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