| 产品编号 | bs-6392R |
| 英文名称 | PCNP Rabbit pAb |
| 中文名称 | PEST含核蛋白抗体 |
| 别 名 | PCNP; PCNP_HUMAN; PEST containing nuclear protein; PEST proteolytic signal containing nuclear protein; PEST proteolytic signal-containing nuclear protein; PEST-containing nuclear protein. |
| 研究领域 | 细胞生物 染色质和核信号 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCNP: 21-120/178 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PCNP is a novel 178 amino acid nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. PCNP is ubiquitinated post-translationally by NIRF (Np95/ICBP90-like RING finger protein), a ubiquitin ligase. Existing as three isoforms produced by alternative splicing events, PCNP is encoded by a gene mapping to human chromosome 3q12.3. Chromosome 3 houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3 Function: May be involved in cell cycle regulation. Subunit: Interacts with UHRF2/NIRF. Post-translational modifications: Ubiquitinated; mediated by UHRF2 and leading to its subsequent proteasomal degradation. SWISS: Q8WW12 Gene ID: 57092 Database links: Entrez Gene: 57092 Human Entrez Gene: 76302 Mouse SwissProt: Q8WW12 Human SwissProt: Q6P8I4 Mouse Unigene: 275865 Human Unigene: 202642 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNP) Polyclonal Antibody, Unconjugated (bs-6392R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
