| 产品编号 | bs-7697R |
| 英文名称 | BS69 Rabbit pAb |
| 中文名称 | 腺病毒5结合蛋白BS69/骨形态发生蛋白受体相关分子1抗体 |
| 别 名 | Adenovirus 5 E1A binding protein; Bone morphogenetic protein receptor associated molecule 1; BRAM1; BS69 variant 1; BS69 variant 2; BS69 variant 3; BS69 variant 4; Zinc finger MYND domain containing 11; ZMYND11. |
| 研究领域 | 细胞生物 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Sheep,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZMYND11/BS69: 501-602/602 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: Corepressor of transcription (Probable). May be involved in chromatin remodeling through association with several remodeling factors. Subunit: Interacts (via MYND-type zinc finger) with NCOR1. Interacts (via MYND-type zinc finger) with human adenovirus early E1A protein (via PXLXP motif); this interaction inhibits E1A mediated transactivation. Interacts (via MYND-type zinc finger) with Epstein-Barr virus EBNA2 protein (via PXLXP motif). Interacts (via MYND-type zinc finger) with EZH2. Interacts with E2F6. Subcellular Location: Nucleus. Chromosome. Note=Associates with chromatin and mitotic chromosomes. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated, leading to proteasomal degradation. Similarity: Contains 1 bromo domain. Contains 1 MYND-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. SWISS: Q15326 Gene ID: 10771 Database links: Entrez Gene: 10771 Human Entrez Gene: 66505 Mouse GenBank: NP_006615 Human GenBank: NP_997644 Human Omim: 608668 Human SwissProt: Q15326 Human SwissProt: Q8R5C8 Mouse Unigene: 292265 Human Unigene: 397307 Mouse Unigene: 38301 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BS69/Adenovirus 5 E1A binding protein Polyclonal Antibody, Unconjugated (bs-7697R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
