| 产品编号 | bs-7713R |
| 英文名称 | Rabbit Anti-MIS12 antibody |
| 中文名称 | 染色体及有丝分裂蛋白MIS12抗体 |
| 别 名 | hMIS12; homolog of yeast Mis12; KNTC2AP; MIND kinetochore complex component, homolog (yeast); MIS12 homolog (yeast); MIS12 homolog; MIS12 MIND kinetochore complex component homolog (yeast); MTW 1; MTW1; Protein MIS12 homolog; MIS12_HUMAN. |
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Specific References (3) | bs-7713R has been referenced in 3 publications.
[IF=12.511] Ogasawara et al. Cathepsin K activity controls cardiotoxin-induced skeletal muscle repair in mice. (2018) J.Cachexia.Sarcopenia.Muscle. 9:160-175 IHC-P ; Mouse.
[IF=9.6] Ogasawara, Shinyu, et al. "Cathepsin K activity controls cardiotoxin‐induced skeletal muscle repair in mice." Journal of Cachexia, Sarcopenia and Muscle (2017). IHC-P ; Mouse.
[IF=5.443] Goto Hiroki. et al. Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice. Stem Cells Int. 2021;2021:9202990 IF ; Mouse.
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| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 24kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MIS12: 11-120/205 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MIS12 is part of a complex that plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly. Function: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Subunit: Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with CASC5, CBX3, CBX5, NDC80 and ZWINT. Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore. Similarity: Belongs to the mis12 family. SWISS: Q9H081 Gene ID: 79003 Database links: UniProtKB/Swiss-Prot: Q9H081.1 细胞周期是细胞最基本的生命活动之一,它通过一系列有序的反应进行分裂,产生两个包含相同遗传物质的子细胞。细胞周期中最核心的事件就是进行遗传物质的复制和分离,即在S期进行DNA的复制,然后在有丝分裂时期将复制的染色体平均分配到子细胞中去。有丝分裂过程中染色体的运动和分离又是通过纺锤体微管和位于染色体着丝粒上的动点之间的协同作用来完成的。 MIS12是一个进化上较为保守的蛋白复合物,人源MIS12复合物由Mis13、Mis14、Mis12和PMF1四个蛋白组成,功能性研究表明它们在动点的组装和染色体的分离中发挥重要作用,但是，目前它们动点定位机制仍不清楚，还有待进一步研究。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MIS12) Polyclonal Antibody, Unconjugated (bs-7713R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
