| 产品编号 | bs-6378R |
| 英文名称 | EID3 Rabbit pAb |
| 中文名称 | E1A样分化抑制因子3抗体 |
| 别 名 | E1A-like inhibitor of differentiation 3; EID 3; EID-1-like inhibitor of differentiation 3; EID-3; EID3; EID3_HUMAN; EP300 interacting inhibitor of differentiation 3; EP300-interacting inhibitor of differentiation 3; Non-SMC element 4, S. cerevisiae, homolog of, B; NSE4B; NSMCE4B. |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EID3: 65-160/333 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Act as a repressor of nuclear receptor-dependent transcription possibly by interfering with CREBBP-dependent coactivation. May function as a coinhibitor of other CREBBP/EP300-dependent transcription factors.Tissue specificity: Highly expressed in testis. Function: Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Acts as a repressor of nuclear receptor-dependent transcription possibly by interfering with CREBBP-dependent coactivation. May function as a coinhibitor of other CREBBP/EP300-dependent transcription factors. Subunit: Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2; EID3 seems to be a testis-specific subunit. NSMCE1, NSMCE4A or EID3 and NDNL2 probably form a subcomplex that bridges the head domains of the SMC5:SMC6 heterodimer. Homodimer, and heterodimer with EID2 (By similarity). Interacts with the C-terminal region of CREBBP. Subcellular Location: Nucleus. Cytoplasm. Chromosome, telomere (Probable). Note=May shuttle between nucleus and cytoplasm. Tissue Specificity: Highly expressed in testis. Similarity: Belongs to the NSE4 family. SWISS: Q8N140 Gene ID: 493861 Database links: Entrez Gene: 493861 Human Entrez Gene: 66341 Mouse Omim: 612986 Human SwissProt: Q8N140 Human SwissProt: Q3V124 Mouse Unigene: 659857 Human Unigene: 443284 Mouse Unigene: 206488 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
