| 产品编号 | bs-7685R |
| 英文名称 | Corin Rabbit pAb |
| 中文名称 | 心脏特异性丝氨酸蛋白酶抗体 |
| 别 名 | ATC 2; ATC2; Atrial natriuteric peptide converting enzyme; Corin serine peptidase; CRN; Heart specific serine proteinase; Heart specific serine proteinase ATC2; Lrp 4; Lrp4; Pro ANP convertase; Pro ANP converting enzyme; TMPRSS 10; Transmembrane protease serine 10. |
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Specific References (2) | bs-7685R has been referenced in 2 publications.
[IF=2.43] Miyazaki et al. Increased levels of soluble corin in pre-eclampsia and fetal growth restriction. (2016) Placenta. 48:20-25 IHC-P ; Human.
[IF=0] Sari, Agnes Rosarina Prita, et al. "Characterization of ovine dermal papilla cell aggregation." International Journal of Trichology 8.3 (2016): 121. IF(ICC) ; Sheep.
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| 研究领域 | 心血管 细胞生物 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Dog,Horse) |
| 产品应用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 115 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Corin: 165-245/1042 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Corin was first described as a trypsin-like serine proteinase in the heart which processed pro-atrial natriuretic peptide into the active form ANP. Corin cleaves pro-ANP at Arg98/Ser-99, and blocking Corin message inhibits pro-ANP processing. Null mutant mice lacking Corin develop hypertension, and a series of missense mutations have been identified in humans with high blood pressure and hypertension. Corin is a member of the type-II transmembrane serine proteinases, such as Hepsin, Epitheliasin, Matriptase and enteropeptidase. The Corin domain structure includes a cytoplasmic domain, a transmembrane domain, a spacer region, a frizzled-like domain, 5 LDL Receptor-like domains, another frizzled-like domain, 3 more LDLR-like repeats, a SRCR (Scavenger Receptor-Cysteine Rich) domain (also called macrophage scavenger-like domain), and finally the trypsin-like serine proteinase domain. The complex domain structure puts Corin into the “mosaic” TMPRSS family, so named because of the wide variety of domains. Function: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Isoform 2: has weaker endopeptidase activity compared to isoform 1. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Isoform 2: Cell membrane; Single-pass type II membrane protein. Note=Less efficiently targeted to the cell membrane compared to isoform 1. Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment: Secreted. Note=Soluble form produced following cleavage by ADAM10. Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment: Secreted. Note=Soluble form produced following autocatalytic cleavage. Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment: Secreted. Note=Soluble form produced following autocatalytic cleavage. Tissue Specificity: Highly expressed in heart. Expressed in heart myocytes. Also expressed in pregnant uterus. Post-translational modifications: N-glycosylated; required for processing and activation. Similarity: Belongs to the peptidase S1 family. Contains 2 FZ (frizzled) domains. Contains 7 LDL-receptor class A domains. Contains 1 peptidase S1 domain. Contains 1 SRCR domain. SWISS: Q9Y5Q5 Gene ID: 10699 Database links: Entrez Gene: 10699 Human Entrez Gene: 53419 Mouse Omim: 605236 Human SwissProt: Q9Y5Q5 Human SwissProt: Q566K6 Mouse SwissProt: Q9Z319 Mouse Unigene: 518618 Human Unigene: 332425 Mouse Unigene: 211679 Rat |
| 产品图片 |
Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-Corin antibody (bs-7685R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
