| 产品编号 | bs-7683R |
| 英文名称 | TReP132 |
| 中文名称 | 乳腺癌抗雌激素抵抗蛋白2抗体 |
| 别 名 | BCAR2; Breast cancer anti estrogen resistance 2; Breast cancer anti-estrogen resistance 2; RAPA; rapa 1; rapa 2; rapa1; rapa2; RP1 139D8.5; Transcriptional regulating factor 1; Transcriptional regulating protein 132; Transcriptional-regulating factor 1; Transcriptional-regulating protein 132; TREF1_HUMAN; TReP 132; TREP132; TRERF1; Zinc finger protein rapa; Zinc finger transcription factor TReP 132; Zinc finger transcription factor TReP-132. |
| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 132kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TReP132/RAPA: 101-200/1200 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1.
Tissue specificity:Highest expression was seen in thymus, testis and adrenal cortex, expressed also in the adrenal medulla, thyroid, and stomach. Highly expressed in steroidogenic JEG-3 and MCF-7 cells, low expression was seen in non-steroidogenic HepG2 and HK293 cells. Function: Activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. Subunit: Interacts with CREBBP and EP300. Subcellular Location: Nucleus. Tissue Specificity: Highest expression was seen in thymus, testis and adrenal cortex, expressed also in the adrenal medulla, thyroid, and stomach. Highly expressed in steroidogenic JEG-3 and MCF-7 cells, low expression was seen in non-steroidogenic Hep-G2 and HEK293 cells. Similarity: Contains 3 C2H2-type zinc fingers. Contains 1 ELM2 domain. Contains 1 SANT domain. SWISS: Q96PN7 Gene ID: 55809 Database links: Entrez Gene: 55809 Human Entrez Gene: 224829 Mouse Omim: 610322 Human SwissProt: Q96PN7 Human SwissProt: Q8BXJ2 Mouse Unigene: 485392 Human Unigene: 260989 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
