| 产品编号 | bs-7668R |
| 英文名称 | Rabbit Anti-GPR65 antibody |
| 中文名称 | G蛋白偶联受体65抗体 |
| 别 名 | GPCR GPR65; Dig1; G protein-coupled receptor 65; Gpcr25; GPR65; hTDAG8; Psychosine receptor; T cell death associated gene 8 protein; T-cell death-associated gene 8; TDAG8. |
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Specific References (2) | bs-7668R has been referenced in 2 publications.
[IF=7.396] Daichi Mori. et al. Extracellular acidity in tumor tissue upregulates programmed cell death protein 1 expression on tumor cells via proton-sensing G protein-coupled receptors. 2021 Sep 13 FC ; mouse.
[IF=5.193] Bortell et al. Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro. (2017) J.Neuroinflammatio. 14:49 ICC,WB ; Rat.
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| 研究领域 | 细胞生物 信号转导 细胞凋亡 细胞周期蛋白 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR65: 51-120/337 <Extracellular> |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GPR65 is a member of the G protein coupled receptor family. It has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. The ligand for this protein is psychosine. GPR65 may have a role in activation-induced cell death or differentiation of T cells. Function: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation-induced cell death or differentiation of T-cells. Subcellular Location: Cell membrane. Tissue Specificity: Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q8IYL9 Gene ID: 8477 Database links: Entrez Gene: 8477 Human Entrez Gene: 14744 Mouse Omim: 604620 Human SwissProt: Q8IYL9 Human SwissProt: Q61038 Mouse Unigene: 443243 Human Unigene: 378924 Mouse |
| 产品图片 |
Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-GPR65 (bs-7668R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 44 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
