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Rabbit Anti-ICAM4  antibody (bs-7637R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购QQ:  400-901-9800
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7637R
英文名称 ICAM4
中文名称 细胞间粘附分子4抗体
别    名 CD242; CD242 antigen; ICAM-4; Icam4; ICAM4_HUMAN; Intercellular adhesion molecule 4; Landsteiner Wiener blood group glycoprotein; Landsteiner-Wiener blood group glycoprotein; LW; LW blood group protein.  
Specific References  (1)     |     bs-7637R has been referenced in 1 publications.
[IF=3.23] Polin, Helene, et al. "Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex." Transfusion 56.4 (2016): 950-955.  FCM ;  Human.  
研究领域 心血管  细胞生物  免疫学  信号转导  细胞凋亡  细胞粘附分子  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse (predicted: Rat,Dog,Pig,Cow,Horse)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 27kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ICAM4: 85-180/271 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.

Subcellular Location:
Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted.

Tissue Specificity:
Erythrocytes.

Post-translational modifications:
N- and O-glycosylated.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
Q14773

Gene ID:
3386

Database links:

Entrez Gene: 3386 Human

Entrez Gene: 78369 Mouse

Entrez Gene: 298702 Rat

Omim: 614088 Human

SwissProt: Q14773 Human

SwissProt: Q9ERM2 Mouse

Unigene: 706750 Human

Unigene: 30220 Mouse

Unigene: 222410 Rat



产品图片
Sample: placenta(Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-ICAM4 (bs-7637R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 45 kD
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-ICAM4 antibody (bs-7637R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-7637R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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