| 产品编号 | bs-2054R |
| 英文名称 | Nkx2.5 Rabbit pAb |
| 中文名称 | 心脏特异性同源盒转录因子NKX2.5抗体 |
| 别 名 | Cardiac-specific homeobox 1; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX; CSX1; Homeobox protein CSX; Homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5(Drosophila); Nkx2-5; NKX2.5; Nkx2E; NKX4-1; Tinman paralog. |
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Specific References (2) | bs-2054R has been referenced in 2 publications.
[IF=2.649] Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10. WB ; Mouse.
[IF=1.86] Ruan, Zhong-Bao, et al. "Inhibitor of p53–p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." Cytotechnology (2015): 1-9. IF(ICC) ; Human.
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| 研究领域 | 心血管 细胞生物 发育生物学 神经生物学 干细胞 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nkx2.5: 85-180/324 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In humans, mutations of the gene encoding the homeobox transcription factor Nkx2.5 result in electrical conduction defects and morphological abnormalities of the heart (Dupays et al., 2005). In the heart, Nkx2.5 is expressed in both the myocardium and the endocardium. Differentiation of embryonic stem cells to Nkx2.5-positive cardiomyocytes is facilitated by Wnt11. Subcellular Location: Nuclear SWISS: P52952 Gene ID: 1482 Database links: Clone ID: 396073 Chicken Entrez Gene: 1482 Human Entrez Gene: 18091 Mouse Omim: 600584 Human SwissProt: Q90788 Chicken SwissProt: P52952 Human SwissProt: P42582 Mouse Unigene: 638 Chicken Unigene: 54473 Human Unigene: 41974 Mouse Unigene: 6179 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; An
Tissue/cell: Rat heart; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
