| 产品编号 | bs-0296G-Cy5 |
| 英文名称 | Goat Anti-Mouse IgG H&L antibody |
| 中文名称 | Cy5标记的羊抗小鼠IgG H&L |
| 别 名 | Goat Anti-Mouse IgG H&L (Cy5); Immunoglobulin G; |
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Specific References (5) | bs-0296G-Cy5 has been referenced in 5 publications.
[IF=9.995] Huijuan Tan. et al. Phase separation of SGS3 drives siRNA body formation and promotes endogenous gene silencing. CELL REP. 2023 Jan;42:111985 IF ; Arabidopsis seedlings,yeast.
[IF=5.869] Qianqian Guo . et al. Heterologous prime-boost immunization co-targeting dual antigens inhibit tumor growth and relapse. Oncoimmunology. 2020;9(1):1841392 ICC ; Hamster.
[IF=5.215] Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243 FCM ; Human.
[IF=4.486] Shen L et al. FPS-ZM1 Alleviates Neuroinflammation in Focal Cerebral Ischemia Rats via Blocking Ligand/RAGE/DIAPH1 PathwayACS Chem Neurosci.2021 Jan 6;12(1):63-78. IF ;
[IF=4.021] Jiang X et al. Overexpression of augmenter of liver regeneration (ALR) mitigates the effect of H 2 O 2-induced endoplasmic reticulum stress in renal tubule epithelial cells. Apoptosis.2019. ICF ; Mouse.
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| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | Flow-Cyt=1:100-1000,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 2.0 mg/ml |
| 免 疫 原 | Native Mouse IgG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Purple, bs-0296G-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
