| 产品编号 | bs-7076R |
| 英文名称 | BMP11 Rabbit pAb |
| 中文名称 | 骨形态发生蛋白11抗体 |
| 别 名 | GDF11; BMP 11; Bone morphogenetic protein 11; GDF 11; Growth differentiation factor 11; Growth/differentiation factor 11; GDF11_HUMAN. |
 |
Specific References (2) | bs-7076R has been referenced in 2 publications.
[IF=4.522] Wang Y et al. Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway. J Cell Physiol. 2019 Oct 21. WB ; Rat.
[IF=2.728] Xijuan Liu et al. Chondrocyte suppression is mediated by miR‐129‐5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572. WB/IHC ; Rabbit.
|
| 研究领域 | 细胞生物 神经生物学 信号转导 生长因子和激素 转录调节因子 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/43 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP11: 301-407/407 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]. Function: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted (Probable). Similarity: Belongs to the TGF-beta family. SWISS: O95390 Gene ID: 10220 Database links: Entrez Gene: 10220 Human Entrez Gene: 14561 Mouse Omim: 603936 Human SwissProt: O95390 Human SwissProt: Q9Z1W4 Mouse Unigene: 600883 Human Unigene: 299218 Mouse Unigene: 51292 Rat |
| 产品图片 |
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-BMP11 (bs-7076R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 12 kD
Observed band size: 12 kD
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP11) Polyclonal Antibody, Unconjugated (bs-7076R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
