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Goat Anti-Mouse IgG H&L / HRP  antibody (bs-0296G-HRP)
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说明书: 100ul  1ml  
100ul/180.00元
1ml/1200.00元
大包装/询价

产品编号 bs-0296G-HRP
英文名称 Goat Anti-Mouse IgG H&L / HRP
中文名称 辣根过氧化物酶标记的羊抗小鼠IgG H&L
别    名 Goat Anti-Mouse IgG H&L (HRP); Immunoglobulin G;   
Specific References  (306)     |     bs-0296G-HRP has been referenced in 306 publications.
[IF=19.568] Jinman Kim. et al. Furin cleavage is required for swine acute diarrhea syndrome coronavirus spike protein-mediated cell–cell fusion. EMERG MICROBES INFEC. 2022 Aug 17  WB ;  Human.  
[IF=16.874] Bingcheng Yi. et al. Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing. BIOACT MATER. 2022 Jul;:  IHC ;  Human.  
[IF=16.744] Fumei He. et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery. CHEM ENG J. 2023 Jan;451:138737  IHC ;  Rat.  
[IF=13.391] Haitao Wang. et al. A plant virus hijacks phosphatidylinositol-3,5-bisphosphate to escape autophagic degradation in its insect vector. AUTOPHAGY. 2022 Sep 10  ELISA ;  Laodelphax striatellus,Insect.  
[IF=12.336] Fuyan Wang. et al. Decellularized porcine cornea-derived hydrogels for the regeneration of epithelium and stroma in focal corneal defects. Ocul Surf. 2020 Oct;18:748  WB ;  Human.  
[IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339  WB ;  Mouse,Human.  
[IF=10.787] Xiaozhen Dai. et al. Nrf2 transcriptional upregulation of IDH2 to tune mitochondrial dynamics and rescue angiogenic function of diabetic EPCs. REDOX BIOL. 2022 Oct;56:102449  WB ;  Mouse.  
[IF=10.103] Jia Qi Liang. et al. miRNAs derived from milk small extracellular vesicles inhibit porcine epidemic diarrhea virus infection. ANTIVIR RES. 2023 Mar;:105579  WB ;  Pig.  
[IF=10.041] Bingcheng Yi. et al. Lysine-doped polydopamine coating enhances antithrombogenicity and endothelialization of an electrospun aligned fibrous vascular graft. Appl Mater Today. 2021 Dec;25:101198  other ;  Rabbit .  
[IF=9.587] Xia, Pengcheng. et al. MicroRNA-22-3p ameliorates Alzheimer’s disease by targeting SOX9 through the NF-κB signaling pathway in the hippocampus. J NEUROINFLAMM. 2022 Dec;19(1):1-19  IHC ;  Mouse.  
[IF=9.221] Rui Chen. et al. Tyramine signal amplification on polystyrene microspheres for highly sensitive quantification of Aflatoxin B1 in peanut samples. SENSOR ACTUAT B-CHEM. 2022 Dec;:133120  Peanut.  
[IF=9.038] Junpeng Zhao. et al. Horseradish peroxidase-catalyzed formation of polydopamine for ultra-sensitive magnetic relaxation sensing of aflatoxin B1. J Hazard Mater. 2021 Jun;:126403  ELISA ;  
[IF=8.786] Wuyi Zeng. et al. A Novel PD-L1-Containing MSLN Targeting Vaccine for Lung Cancer Immunotherapy. FRONT IMMUNOL. 2022; 13: 925217  ELISA ;  Mouse.  
[IF=8.724] Qing Ma. et al. Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents. Bioact Mater. 2021 Dec;6:4786  Other ;  
[IF=8.44] Shi Xiaobo. et al. miR-4443 promotes radiation resistance of esophageal squamous cell carcinoma via targeting PTPRJ. J TRANSL MED. 2022 Dec;20(1):1-14  WB ;  Human.  
[IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268.  WB ;  Mouse.  
[IF=8.101] Shuang Xie. et al. Indispensable role of melatonin, a scavenger of reactive oxygen species (ROS), in the protective effect of Akkermansia muciniphila in cadmium-induced intestinal mucosal damage. FREE RADICAL BIO MED. 2022 Nov;193:447  WB ;  Mouse.  
[IF=8.039] Xin-Chen Jiang. et al. Discovery of a Novel G-Quadruplex and Histone Deacetylase (HDAC) Dual-Targeting Agent for the Treatment of Triple-Negative Breast Cancer. J MED CHEM. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse, Human.  
[IF=8.025] Ziyu Liu. et al. Exosomes from LSD1 knockdown breast cancer cells activate osteoclastogenesis and inhibit osteoblastogenesis. INT J BIOL MACROMOL. 2023 Feb;:123792  WB ;  Human,Mouse.  
[IF=8.022] Aixin Ni. et al. Degradation of amyloid β-peptides catalyzed by nattokinase in vivo and in vitro. FOOD SCI HUM WELL. 2023 Sep;12:1905  WB ;  Escherichia coli.  
[IF=7.701] Tian, Hongwei. et al. Centromeric protein K (CENPK) promotes gastric cancer proliferation and migration via interacting with XRCC5. GASTRIC CANCER. 2022 Jun;:1-17  WB ;  Human.  
[IF=7.666] Chaoyue Zhong. et al. Establishment of a Spermatogonial Stem Cell Line with Potential of Meiosis in a Hermaphroditic Fish, Epinephelus coioides. CELLS-BASEL. 2022 Jan;11(18):2868  WB, IF ;  Fish.  
[IF=7.561] Niu H. et al. Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice.. Front Immunol. 2021 Dec;12:800965-800965  WB ;  Recombinant L. plantarum.  
[IF=7.425] Xiaobo Hu. et al. Polydopamine-mediated quantity-based magnetic relaxation sensing for the rapid and sensitive detection of chloramphenicol in fish samples. FOOD RES INT. 2022 Sep;:111919  Other ;  Other.  
[IF=7.419] Xueqi Chen. et al. GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes. BIOMED PHARMACOTHER. 2023 Jun;162:114694  WB ;  Mouse.  
[IF=7.419] Qin Zhang. et al. Relief of ovalbumin-induced airway remodeling by the glycyl-l-histidyl-l-lysine-Cu2+ tripeptide complex via activation of SIRT1 in airway epithelial cells. BIOMED PHARMACOTHER. 2023 Aug;164:114936  WB ;  Mouse,Human.  
[IF=7.333] Homogenous Magneto-Fluorescent Nanosensor for Tumor-Derived Exosomes Isolation and Analysiset al. Homogenous Magneto-Fluorescent Nanosensor for Tumor-Derived Exosomes Isolation and Analysis. ACS Sens . 2020 Jul 24;5(7):2052-2060.  FCM ;  Human.  
[IF=7.171] Yawen Bu. et al. YLMY Tyrosine Residue within the Cytoplasmic Tail of Newcastle Disease Virus Fusion Protein Regulates Its Surface Expression to Modulate Viral Budding and Pathogenicity | Microbiology Spectrum. Microbiol Spectr. 2021 Dec;:  WB ;  Hamster.  
[IF=7.169] Ma, Yan-hong. et al. Inhibiting the transcription and replication of Ebola viruses by disrupting the nucleoprotein and VP30 protein interaction with small molecules. ACTA PHARMACOL SIN. 2023 Feb;:1-13  WB ;  Human.  
[IF=7.003] Chen et al. A novel Meloidogyne graminicola effector, MgGPP, is secreted into host cells and undergoes glycosylation in concert with proteolysis to suppress plant defenses and promote parasitism. (2017) PLoS.Patho. 13:e1006301  WB ;  Mouse.  
抗体来源 Goat
克隆类型 Polyclonal
交叉反应 Rat,Mouse,Human
产品应用 WB=1:10000-50000, IHC-P=1:200, ELISA=1:50000-200000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状 Liquid
浓    度 2.0 mg/ml
免 疫 原 Native Mouse IgG 
亚    型 IgG
纯化方法 affinity purified by Protein G, nonspecific adsorbed
缓 冲 液 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

产品图片
Sample: Lane 1,3,5 : Rat Cerebrum tissue lysates Lane 2,4,6 : Human MCF-7 cell lysates Primary: Anti- GAPDH (bsm-33033M) at 1/5000 dilution Secondary: Goat Anti-Mouse IgG H&L-HRP (bs-0296G-HRP) at 1/20000~1/100000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Lamin B (Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33442M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA(Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:200) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (bsm-33060M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024 bs-0296G-HRP 1:300) instructionsand DAB staining.
Immobilized mouse IgG (bs-0296P, nothing else) at 0.2μg/well can bind Goat Anti-Mouse IgG H&L/HRP antibody (Cat. No. bs-0296G-HRP).
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