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Goat Anti-Rabbit IgG H&L antibody (bs-0295G-HRP)

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说明书： 100ul 1ml

100ul/180.00元

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1ml/1200.00元

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产品编号	bs-0295G-HRP
英文名称	Goat Anti-Rabbit IgG H&L antibody
中文名称	辣根过氧化物酶标记的羊抗兔IgG H&L
别名	Goat Anti-Rabbit IgG H&L (HRP); Immunoglobulin G;
	Specific References (757) \| bs-0295G-HRP has been referenced in 757 publications. [IF=20.693] Pei Dai. et al. Gimap5 promoted RSV degradation through interaction with M6PR. J MED VIROL. 2022 Dec;: WB ; Human. PubMed:36484389 [IF=18.027] Nguyen Thi Nguyen. et al. Amplified Fenton-Based Oxidative Stress Utilizing Ultraviolet Upconversion Luminescence-Fueled Nanoreactors for Apoptosis-Strengthened Ferroptosis Anticancer Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:36579941 [IF=18.027] Guanghao Wu. et al. Enhanced Proliferation of Visualizable Mesenchymal Stem Cell–Platelet Hybrid Cell for Versatile Intracerebral Hemorrhage Treatment. ACS NANO. 2023;XXXX(XXX):XXX-XXX WB ; PubMed:37037487 [IF=17.694] Chen, Jiyun. et al. Structural basis for MTA1c-mediated DNA N6-adenine methylation. NAT COMMUN. 2022 Jun;13(1):1-15 WB ; Substrate DNA binding by the MTA1c complex. PubMed:35672411 [IF=16.806] Zhenjie Wang. et al. Dynamic Adjust of Non-Radiative and Radiative Attenuation of AIE Molecules Reinforces NIR-II Imaging Mediated Photothermal Therapy and Immunotherapy. 2022 Jan 22 Other ; Other. PubMed:35064653 [IF=16.744] Zhanlin Zhang. et al. Persistent luminescence-activated Janus nanomotors with integration of photodynamic and photothermal cancer therapies. CHEM ENG J. 2022 Dec;:141226 IHC ; Mouse. PubMed:10.1016/j.cej.2022.141226 [IF=16.744] Junjie Feng. et al. Rapid and efficient fluorescent aptasensor for PD-L1 positive extracellular vesicles isolation and analysis: EV-ANCHOR. CHEM ENG J. 2023 Apr;:142811 WB ; Human. PubMed:10.1016/j.cej.2023.142811 [IF=15.304] Zhenjie Wang. et al. Phototheranostic nanoparticles with aggregation-induced emission as a four-modal imaging platform for image-guided photothermal therapy and ferroptosis of tumor cells. BIOMATERIALS. 2022 Oct;289:121779 WB ; Human. PubMed:36099712 [IF=14.588] Xuliang Guo. et al. Electron-Accepting Micelles Deplete Reduced Nicotinamide Adenine Dinucleotide Phosphate and Impair Two Antioxidant Cascades for Ferroptosis-Induced Tumor Eradication. Acs Nano. 2020;14(11):14715–14730 WB ; Mouse. PubMed:33156626 [IF=13.903] Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5. WB ; Rabbit. PubMed:31689086 [IF=13.391] Haitao Wang. et al. A plant virus hijacks phosphatidylinositol-3,5-bisphosphate to escape autophagic degradation in its insect vector. AUTOPHAGY. 2022 Sep 10 ELISA ; Laodelphax striatellus,Insect. PubMed:36093594 [IF=12.336] Fuyan Wang. et al. Decellularized porcine cornea-derived hydrogels for the regeneration of epithelium and stroma in focal corneal defects. Ocul Surf. 2020 Oct;18:748 WB ; Human. PubMed:32841745 [IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339 WB ; Mouse,Human. PubMed:34987648 [IF=11.467] Jin Deng. et al. Modified CFBP-bFGF targeting to ischemic brain promoted the functional recovery of cerebral ischemia. J CONTROL RELEASE. 2023 Jan;353:462 WB ; Rat. PubMed:36493946 [IF=11.151] Qijing Wu. et al. Remodeling Chondroitin-6-Sulfate–Mediated Immune Exclusion Enhances Anti–PD-1 Response in Colorectal Cancer with Microsatellite Stability. Cancer Immunol Res. 2021 Dec;: IHC ; Human. PubMed:34933913 [IF=10.723] Yao Lin. et al. Drug-free and non-crosslinked chitosan/hyaluronic acid hybrid hydrogel for synergistic healing of infected diabetic wounds. CARBOHYD POLYM. 2023 Aug;314:120962 IHC ; Mouse. PubMed:37173016 [IF=10.711] Zihuan Luo. et al. Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection. BIOENG TRANSL MED. 2022 Apr 06 IHC ; Rat. PubMed:10.1002/btm2.10316 [IF=10.435] Wang, Congcong. et al. Engineering a HEK-293T exosome-based delivery platform for efficient tumor-targeting chemotherapy/internal irradiation combination therapy. J NANOBIOTECHNOL. 2022 Dec;20(1):1-17 WB ; Human. PubMed:35642064 [IF=10.269] Zhang Yufei. et al. LAT1 targeted brain delivery of temozolomide and sorafenib for effective glioma therapy. NANO RES. 2023 Apr;:1-9 PubMed:10.1007/s12274-023-5568-3 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119 WB ; Mouse. PubMed:36109005 [IF=10.103] Jia Qi Liang. et al. miRNAs derived from milk small extracellular vesicles inhibit porcine epidemic diarrhea virus infection. ANTIVIR RES. 2023 Mar;:105579 WB ; Pig. PubMed:36907442 [IF=9.587] Luo, Lu. et al. Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice. J NEUROINFLAMM. 2022 Dec;19(1):1-27 WB ; Mouse. PubMed:35690810 [IF=9.434] Bingting Chen. et al. Glycoursodeoxycholic acid regulates bile acids level and alters gut microbiota and glycolipid metabolism to attenuate diabetes. GUT MICROBES. 2023;15(1):Article: 2192155 WB ; Mouse. PubMed:36967529 [IF=9.417] Nanhang Zhu. et al. Self-calibrated magnetic aptamer sensor with dual Lanthanide-assisted Time-resolved luminescence for high-sensitive detection of melanoma exosomal PD-L1. MATER DESIGN. 2023 Mar;227:111714 WB ; Mouse. PubMed:10.1016/j.matdes.2023.111714 [IF=8.947] Zekai Cui. et al. LM22B-10 promotes corneal nerve regeneration through in vitro 3D co-culture model and in vivo corneal injury model. ACTA BIOMATER. 2022 May;: WB ; Chicken. PubMed:35562005 [IF=8.943] Yujiao He. et al. Polystyrene nanoplastics deteriorate LPS-modulated duodenal permeability and inflammation in mice via ROS drived-NF-κB/NLRP3 pathway. CHEMOSPHERE. 2022 Nov;307:135662 WB, IHC ; Mouse, Human. PubMed:35830933 [IF=8.786] Shaoqiu Leng. et al. Ion channel Piezo1 activation promotes aerobic glycolysis in macrophages.. FRONT IMMUNOL. 2022 Sep;13:976482-976482 IHC ; Mouse. PubMed:36119083 [IF=8.712] Yu et al. Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration. (2017) Theranostics. 7:3387-3397 IHC ; Rabbit. PubMed:28900517 [IF=8.44] Shi Xiaobo. et al. miR-4443 promotes radiation resistance of esophageal squamous cell carcinoma via targeting PTPRJ. J TRANSL MED. 2022 Dec;20(1):1-14 WB ; Human. PubMed:36578050 [IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268. WB ; Rabbit. PubMed:doi:10.1016/j.cej.2020.124268
抗体来源	Goat
克隆类型	Polyclonal
交叉反应	Rabbit
产品应用	WB=1:2000-20000,IHC-P=1:200-1000,IHC-F=1:200-1000,ELISA=1:2000-20000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性状	Liquid
浓度	2.0 mg/ml
免疫原	Native rabbit IgG
亚型	IgG
纯化方法	affinity purified by Protein G, nonspecific adsorbed
缓冲液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
产品图片	Sample: Lane 1: SH-SY5Y (Human) Cell Lysate Lane 2: MCF-7 (Human) Cell Lysate Primary: Anti-GAPDH (bs-10900R) at 1/20000 dilution Secondary: Goat Anti-Rabbit IgG H&L-HRP at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated (bs-46001R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining. Immobilized rabbit IgG (bs-0295P, nothing else) at 0.2μg/well can bind Goat Anti-Rabbit IgG H&L/HRP antibody (Cat. No. bs-0295G-HRP).

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序