| 产品编号 | bs-6769R |
| 英文名称 | PHAPI2 Rabbit pAb |
| 中文名称 | 酸性核磷蛋白32家族B抗体 |
| 别 名 | APRIL; PHAPI2; SSP29; 2410015B15Rik; PAL31; PHAPI2a; AN32B_HUMAN; ANP32B; Acidic protein rich in leucines; Putative HLA-DR-associated protein I-2 (PHAPI2); Silver-stainable protein SSP29; AN32B_MOUSE; Proliferation-related acidic leucine-rich protein PAL3 |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Sheep,Cow,Zebrafish,Dog,Fruit Fly) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PHAPI2/APRIL: 51-150/251 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Function: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Subunit: Monomer. Interacts with histones H3 and H4. Subcellular Location: Isoform 1: Nucleus. Note=Accumulates in the nuclei at the S phase (By similarity). Isoform 2: Cytoplasm. Note=Lacks a nuclear localization signal. Tissue Specificity: Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta. Post-translational modifications: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). Similarity: Belongs to the ANP32 family. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. SWISS: Q92688 Gene ID: 10541 Database links: Entrez Gene: 10541 Human Entrez Gene: 67628 Mouse SwissProt: Q92688 Human SwissProt: Q9EST5 Mouse Unigene: 494604 Human Unigene: 263913 Mouse Unigene: 20465 Rat |
| 产品图片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-PHAPI2 (bs-6769R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
