| 产品编号 | bs-7549R |
| 英文名称 | GAS 6 Rabbit pAb |
| 中文名称 | 生长停滞特异性蛋白6抗体 |
| 别 名 | AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; Gas 6; GAS6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor. |
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Specific References (4) | bs-7549R has been referenced in 4 publications.
[IF=8.322] Zhou, Hang. et al. AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. J Neuroinflamm. 2021 Dec;18(1):1-17 WB,IF ; Mouse.
[IF=6.684] Shanyu Fang. et al. NTNG1 Modulates Cisplatin Resistance in Epithelial Ovarian Cancer Cells via the GAS6/AXL/Akt Pathway. Front Cell Dev Biol. 2021; 9: 652325 WB ; Human.
[IF=5.882] Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18 WB ; Human,Mouse.
[IF=4.025] Dai Jing-Cong. et al. GAS6-mediated dialogue between decidual stromal cells and macrophages is essential for early pregnancy maintenance by inducing M2-like polarization and cell proliferation of decidual macrophages. Mol Hum Reprod. 2022 Mar;28(3): IHC ; Human.
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| 研究领域 | 肿瘤 心血管 信号转导 干细胞 细胞凋亡 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 76 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GAS 6: 551-650/721 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GAS 6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS 6 regulates chondrogenic differentiation from mesenchymal cells and also plays a role in thrombosis by amplifying platelet aggregation and secretion in response to known agonists. It is over expressed in human gliomas and regulate the phagocytic function of sertoli cells. Function: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Subunit: Heterodimer and heterotetramer with AXL. Subcellular Location: Secreted. Tissue Specificity: Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. Post-translational modifications: Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Similarity: Contains 4 EGF-like domains. Contains 1 Gla (gamma-carboxy-glutamate) domain. Contains 2 laminin G-like domains. SWISS: Q14393 Gene ID: 2621 Database links: Entrez Gene: 2621 Human Entrez Gene: 14456 Mouse Omim: 600441 Human SwissProt: Q14393 Human SwissProt: Q61592 Mouse Unigene: 646346 Human Unigene: 3982 Mouse GAS6在基因转录调节中起重要的调节作用,参与对细胞存活、增殖、粘附、迁移及抗凋亡的调节，Axl属于受体酪氨酸激酶(receptor tyrosine kinases,RTK)家族成员,为Axl的生物配体。经研究发现；Gas6与多种肿瘤细胞的增殖、存活、血管发生、侵袭有关，Gas6系统具有广泛的生物学作用，Gas6属维生素K依赖的蛋白质,广泛表达于各类细胞。 |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAS 6 (bs-7549R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
