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Rabbit Anti-MBP tag  antibody (bs-2967R)  
~~~促销代码KT202411~~~
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说明书: 100ul  500ul  
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bs-2967R
英文名称 Rabbit Anti-MBP tag  antibody
中文名称 麦芽糖结合蛋白抗体
别    名 MBP-tag; MBP; MMBP; Maltose Binding Protein; Maltose binding protein tag; ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; Periplasmic maltose binding protein; MALE_ECOLI.  
产品类型 标签抗体 
研究领域 细胞生物  免疫学  糖蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Recombinant protein
产品应用 WB=1:5000-20000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 44kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from E. coli Maltose Binding Protein: 1-100/396 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.

Function:
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.

Subcellular Location:
Periplasm.

Similarity:
Belongs to the bacterial solute-binding protein 1 family.

SWISS:
P0AEX9

Gene ID:
1039564

Database links:
Entrez Gene: 1039564 Escherichia coli

Entrez Gene: 4494002 Escherichia coli

Entrez Gene: 948538 Escherichia coli

Entrez Gene: 960089 Escherichia coli

SwissProt: P0AEX9 Escherichia coli

SwissProt: P0AEY0 Escherichia coli



产品图片
Sample: Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) at 4 ug Primary: Anti-MBP tag (bs-2967R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD
Sample: Lane 1: MBP tagged cytochrome C (Full Length) Protein (bs-41232P) at 4 ug Lane 2: MBP tagged caspase 3 p17 (143-213) Protein (bs-41233P) at 4 ug Primary: Anti-MBP tag (bs-2967R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60-70 kD Observed band size: 62 kD
Sample: Lane 1: MBP-Tagged Fusion Protein (bs- 41233P) at 300ng Primary: Anti-MBP tag (bs-2967R) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa
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