| 产品编号 | bs-7517R |
| 英文名称 | Natriuretic Peptide Receptor A Rabbit pAb |
| 中文名称 | 利钠肽受体A抗体 |
| 别 名 | NPRA; ANP A; ANP-A; ANPa; ANPR-A; ANPRA; ANPRA_HUMAN; Atrial natriuretic peptide A type receptor; Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor A; Atrial natriuretic peptide receptor type A; Atrionatriuretic peptide receptor A; GC A; GC-A; Guanylate cyclase A; Guanylate cyclase; GUC2A; GUCY2A; Natriuretic peptide A type receptor; Natriuretic peptide receptor A/guanylate cyclase A; NPR 1; NPR A; NPR-A; NPR1; NPRA. |
| 研究领域 | 心血管 细胞生物 信号转导 G蛋白偶联受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 113 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Natriuretic Peptide Receptor A: 261-360/1061 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009] Function: Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. Subunit: Homodimer. Subcellular Location: Membrane; Single-pass type I membrane protein. Post-translational modifications: Phosphorylation of the protein kinase-like domain is required for full activation by ANP. Similarity: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. Contains 1 protein kinase domain. SWISS: P16066 Gene ID: 4881 Database links: Entrez Gene: 4881 Human Omim: 108960 Human SwissProt: P16066 Human Unigene: 490330 Human Unigene: 10463 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Natriuretic Peptide Receptor A Polyclonal Antibody, Unconjugated (bs-7517R) at 1:200 overni
Paraformaldehyde-fixed, paraffin embedded Human Duodenum ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Natriuretic Peptide Receptor A Polyclonal Antibody, Unconjugated (bs-7517R) at 1:200 overnight at
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
