| 产品编号 | bs-7051R |
| 英文名称 | DENTT Rabbit pAb |
| 中文名称 | 细胞分裂核仁蛋白1抗体 |
| 别 名 | CDA1; CINAP; CTCL; DENTT; HRIHFB2216; NP79; SE204; TSPX; DXBwg1396e; DXHXS1008E; E130307F10Rik; RGD1563283; TSYL2_HUMAN; TSPYL2; TSPY-like protein 2; Cell division autoantigen 1; Cutaneous T-cell lymphoma-associated antigen se20-4 (CTCL-associated antigen |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 76 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DENTT: 251-350/693 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CASK interacting nucleosome assembly protein (CINAP) modulates gene expression and its abundance in cultured neurons is regulated by synaptic activity. CINAP is widely expressed in different regions of adult mouse brain, including the cerebral cortex, hippocampus, striatum, hypothalamus, cerebellum, and two adult brain regions known to generate progenitor neurons. During early development, CINAP is also expressed in regions where neuronal progenitor cells are actively dividing, the ventricular and subventricular zones, suggesting that in addition to regulating gene expression in mature neurons CINAP may also play a role in dividing cells. Function: Part of the CASK/TRB1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression. Subunit: Interacts with histones. Interacts with CASK. Part of a complex containing CASK, TRB1 and TSPYL2. Subcellular Location: Nucleus. Cytoplasm. Note=Enriched in transcriptionally active regions of chromatin in neurons. Tissue Specificity: Ubiquitously expressed, with highest levels in brain, testis and heart, and lowest levels in liver and pancreas. Post-translational modifications: Phosphorylation at Ser-20 and/or Thr-340 impairs function on cell proliferation (Probable). Similarity: Belongs to the nucleosome assembly protein (NAP) family. SWISS: Q9H2G4 Gene ID: 64061 Database links: Entrez Gene: 64061 Human Entrez Gene: 52808 Mouse Omim: 300564 Human SwissProt: Q9H2G4 Human SwissProt: Q7TQI8 Mouse Unigene: 136164 Human Unigene: 592916 Human Unigene: 9997 Mouse |
| 产品图片 |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DENTT) polyclonal Antibody, Unconjugated (bs-7051R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
