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Rabbit Anti-Sprouty 2  antibody (bs-7507R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-7507R
英文名称 Sprouty 2
中文名称 快速发育生长因子同源蛋白2抗体
别    名 hSPRY 2; hSPRY2; MGC23039; Sprouty homolog 2 (Drosophila); Sprouty homolog 2; Sprouty2; Spry 2; Spry2; SPY2_HUMAN.  
研究领域 肿瘤  心血管  细胞生物  神经生物学  干细胞  生长因子和激素  血管内皮细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Rat (predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep)
产品应用 WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 35kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Sprouty 2: 65-170/315 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty 2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C terminus of the protein. Significant sequence divergence occurs at the N terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Function:
May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.

Subunit:
Interacts with RAF1.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Note=Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).

Similarity:
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain.

SWISS:
O43597

Gene ID:
10253

Database links:

Entrez Gene: 10253 Human

Entrez Gene: 24064 Mouse

Entrez Gene: 306141 Rat

Omim: 602466 Human

SwissProt: O43597 Human

SwissProt: Q9QXV8 Mouse

Unigene: 18676 Human

Unigene: 89982 Mouse

Unigene: 103294 Rat



Sprouty2是一类在全身组织广泛表达的软脂酰化磷蛋白,在机体的信号转导,尤其是受体蛋白对受体酪氨酸激酶(RTK)等信号通路起着重要的抑制作用,其表达在多种肿瘤组织中发生明显下降,并可进一步引起细胞异常增殖、转化及新生血管形成,从而促进肿瘤的发生及发展。
产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Sprouty 2 (bs-7507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sprouty 2) Polyclonal Antibody, Unconjugated (bs-7507R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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