| 产品编号 | bs-6787R |
| 英文名称 | Rabbit Anti-SLC26A4 antibody |
| 中文名称 | 钠碘单独转运蛋白SLC26A4抗体 |
| 别 名 | PDS; deafness, autosomal recessive 4; DFNB4; EVA; NSRD4; Pendred syndrome; Pendred syndrome homolog; Pendrin; S26A4_HUMAN; SLC26A4; Sodium independent chloride/iodide transporter; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4. |
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Specific References (2) | bs-6787R has been referenced in 2 publications.
[IF=3.164] Yamauchi T et al. Na+-Cl- cotransporter-mediated chloride uptake contributes to hypertension and renal damage in aldosterone-infused rats.Am J Physiol Renal Physiol. 2018 Aug 1;315(2):F300-F312. WB ; Rat.
[IF=2.765] Andharia et al. Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells. (2018) Pflugers.Arch. 470:897-907 IHC/WB ; guinea pigs.
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| 研究领域 | 细胞生物 神经生物学 信号转导 转录调节因子 通道蛋白 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 93kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Solute carrier family 26 member 4: 301-400/780 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the solute carrier 26 family, whose members encode anion transporter proteins. This particular family member encodes a protein involved in transporting chloride, oxalate, sulfate and bicarbonate. Several alternatively spliced transcript variants of this gene, encoding distinct isoforms, have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]. Function: Sodium-independent transporter of chloride and iodide. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues. DISEASE: Defects in SLC26A4 are the cause of deafness autosomal recessive type 4 (DFNB4) [MIM:600791]; also known as vestibular aqueduct syndrome (EVA). DFNB4 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNB4 is associated with an enlarged vestibular aqueduct. Similarity: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. Contains 1 STAS domain. SWISS: O43511 Gene ID: 5172 Database links: UniProtKB/Swiss-Prot: O43511.1 |
| 产品图片 |
Sample:
Lane 1: Human TT cell lysates
Lane 2: Human U251 cell lysates
Primary: Anti-SLC26A4 (bs-6787R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-SLC26A4 antibody (bs-6787R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (U87MG); Negative blank control (Molt4）
Green line : Primary Antibody (Rabbit Anti-SLC26A4 antibody (bs-6787R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
U87MG（Positive）and Molt4（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC26A4 Antibody(bs-6787R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
