| 产品编号 | bs-6304R |
| 英文名称 | Rabbit Anti-IFNA4 antibody |
| 中文名称 | 干扰素α4抗体 |
| 别 名 | Interferon alpha 4 precursor; Interferon alpha 4B; Interferon alpha 76; Interferon alpha M1; Interferon, alpha 4; IFNA4_HUMAN. |
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Specific References (1) | bs-6304R has been referenced in 1 publications.
[IF=5.714] Xueer Liu. et al. Lidocaine inhibits influenza a virus replication by up-regulating IFNα4 via TBK1-IRF7 and JNK-AP1 signaling pathways. INT IMMUNOPHARMACOL. 2023 Feb;115:109706 WB ; Mouse.
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| 研究领域 | 肿瘤 细胞生物 免疫学 干扰素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IFNA4: 101-189/189 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Produced by macrophages, IFN alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Subcellular Location: Secreted. Similarity: Belongs to the alpha/beta interferon family. SWISS: P05014 Gene ID: 3441 Database links: Entrez Gene: 3441 Human Omim: 147564 Human SwissProt: P05014 Human Unigene: 1510 Human |
| 产品图片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- IFNA4 (bs-6304R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 20 kD
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IFNA4 Polyclonal Antibody, Unconjugated(bs-6304R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
