| 产品编号 | bs-6653R |
| 英文名称 | MX1 |
| 中文名称 | MX1抗体 |
| 别 名 | N-terminally processed; Human interferon regulated resistance GTP binding protein MXA; IFI 78; IFI 78K; IFI-78K; IFI78; IFI78K; Interferon induced GTP binding protein Mx1; Interferon induced protein p78; Interferon inducible protein p78; Interferon regulated resistance GTP binding protein; Interferon regulated resistance GTP binding protein MxA; Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX 1; MX; MX1; MX1_HUMAN; MxA; Myxoma resistance protein 1; Myxovirus (influenza virus) resistance 1 interferon inducible; Myxovirus (influenza virus) resistance 1 interferon inducible protein p78; Myxovirus (influenza) resistance 1 homolog of murine; Myxovirus resistance protein 1. |
| 研究领域 | 细胞生物 微生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Pig,Cow,Horse,Sheep) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 73kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MX1: 231-330/662 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Dynamin family of microtubule-associated proteins function as GTPases that are involved in microtubule bundling and endocytosis. In mice, Mx2 (myxovirus resistance protein 2) and Mx1 (myxovirus resistance protein 1) are members of the Dynamin family that are involved in the immune response to viral infections. Localized to the cytoplasm, Mx2 contains one GED domain and is expressed in response to viral infection or treatment by IFN-alpha/IFN-beta. Once expression is induced, Mx2 accumulates in the cytoplasm and inhibits the replication of vesicular stomatitis virus (VSV), thereby conferring resistance to VSV infection. Unlike Mx2, Mx1 is localized to the nucleus where, upon induction by IFN-alpha/IFN-beta, it provides selective resistance to infection by the highly lethal H5N1 influenza virus. In humans, MxA and MxB function in a similar manner to Mx1 and Mx2, conferring resistance to specific target viruses. Mx3 is a rat-specific member of the myxovirus resistance protein family. Function: May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus. Subunit: Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7. Subcellular Location: Cytoplasm. Secreted, extracellular space. Nucleus. Note=Translocates into the nuclei of HSV-1 infected cells. Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. SWISS: P20591 Gene ID: 4599 Database links: Entrez Gene: 4599 Human Omim: 147150 Human SwissProt: P20591 Human Unigene: 517307 Human |
| 产品图片 |
Tissue/cell: mouse breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFI78/MX1 Polyclonal Antibody, Unconjugated(bs-6653R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
