| 产品编号 | bs-6589R |
| 英文名称 | BCAT2 Rabbit pAb |
| 中文名称 | 支链氨基酸转氨酶2抗体 |
| 别 名 | BCAM; BCATM; BCT2; HVLI; PP18; Bcat(m); Bcat-2; Eca40; BCAT2_HUMAN; BCAT2; Placental protein 18 (PP18); 2.6.1.42; BCAT2_MOUSE; |
 |
Specific References (3) | bs-6589R has been referenced in 3 publications.
[IF=4.079] Jason S. Hinkle. et al. AICAR stimulates mitochondrial biogenesis and BCAA catabolic enzyme expression in C2C12 myotubes. Biochimie. 2021 Nov;: WB ; Mouse.
[IF=2.795] Madison E Rivera et al. Effect of valine on myotube insulin sensitivity and metabolism with and without insulin resistance. Mol Cell Biochem
. 2020 May;468(1-2):169-183. WB ; mouse.
[IF=1.88] Madison E. Rivera. et al. Comparing the effects of palmitate, insulin, and palmitate-insulin co-treatment on myotube metabolism and insulin resistance. 2021 Aug 12 WB ; Mouse.
|
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BCAT2: 101-200/392 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Class-IV pyridoxal-phosphate-dependent aminotransferase family members ECA39 and BCAT2 are both enzymes that catalyze the first reaction in the catabolism of the essential branched chain amino acids valine, leucine and isoleucine. ECA39, also known as BCAT1 (branched-chain-amino-acid aminotransferase 1, cytosolic) is localized to the cytoplasm where it forms a homodimer. ECA39 is expressed in the brain and kidney during embryogenesis and is overexpressed in c-Myc induced tumors. BCAT2 (branched-chain-amino-acid aminotransferase 2, mitochondrial), also known as placental protein 18 (PP18), is expressed as two isoforms produced by alternative splicing. The first isoform of BCAT2, designated BCAT2A, is expressed in the mitochondrion, while the second isoform, designated BCAT2B, is expressed in the cytoplasm. Ubiquitously expressed, BCAT2 is also thought to act as a transporter of branched chain α-keto acids. Function: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Subunit: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Subcellular Location: Cytoplasm and Mitochondrion. Tissue Specificity: Ubiquitous. Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. SWISS: O15382 Gene ID: 587 Database links: Entrez Gene: 587 Human Entrez Gene: 12036 Mouse Omim: 113530 Human SwissProt: O15382 Human SwissProt: O35855 Mouse Unigene: 512670 Human Unigene: 24210 Mouse |
| 产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-BCAT2 (bs-6589R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD
Sample: Kidney (Mouse) Tissue Lysate at 30 ug
Primary: Anti-BCAT2(bs-6589R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BCAT2/BCAM Polyclonal Antibody, Unconjugated(bs-6589R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
