| 产品编号 | bs-6583R |
| 英文名称 | Rabbit Anti-RNASE4 antibody |
| 中文名称 | 核糖核酸酶4抗体 |
| 别 名 | RAB1; Ribonuclease 4; Ribonuclease A B1; Ribonuclease, RNase A family, 4; RNAS4_HUMAN; RNase 4; RNS4. |
| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 血管内皮细胞 细胞骨架 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNASE4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]. Function: This RNase has marked specificity towards the 3' side of uridine nucleotides. Subcellular Location: Secreted. Similarity: Belongs to the pancreatic ribonuclease family. SWISS: P34096 Gene ID: 6038 Database links: Entrez Gene: 6038 Human Entrez Gene: 58809 Mouse SwissProt: P34096 Human SwissProt: Q9JJH1 Mouse |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HUVEC cell lysates
Primary: Anti-RNASE4 (bs-6583R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 15 kDa
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated(bs-6583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
