| 产品编号 | bs-6576R |
| 英文名称 | RBBP6 Rabbit pAb |
| 中文名称 | 视网膜母细胞瘤结合蛋白6抗体 |
| 别 名 | Retinoblastoma binding protein 6; RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; PACT; Proliferationpotentialrelated protein; Protein P2P R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding Q protein 1; RBBP 6; RBQ 1; RBQ1; Ret |
| 研究领域 | 肿瘤 细胞生物 细胞凋亡 细胞周期蛋白 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 201 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBBP6: 1201-1350/1792 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. Retinoblastoma binding protein 6 binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. Function: E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. Subunit: Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6. Subcellular Location: ucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome. Tissue Specificity: Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated by NEK6. Similarity: Contains 1 CCHC-type zinc finger. Contains 1 DWNN domain. Contains 1 RING-type zinc finger. SWISS: Q7Z6E9 Gene ID: 5930 Database links: Entrez Gene: 5930 Human Entrez Gene: 19647 Mouse Omim: 600938 Human SwissProt: Q7Z6E9 Human SwissProt: P97868 Mouse Unigene: 188553 Human Unigene: 4480 Mouse Unigene: 95305 Rat |
| 产品图片 |
Tissue/cell: human Placenta; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RBBP6, Unconjugated(bs-6576R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
