| 产品编号 | bs-6574R |
| 英文名称 | RRBP1 Rabbit pAb |
| 中文名称 | 核糖体结合蛋白1抗体 |
| 别 名 | 180 kDa ribosome receptor homolog; DKFZp586A1420; ES/130; ES/130 related protein; ES130; FLJ36146; hES; Ribosome binding protein 1; Ribosome binding protein 1 homolog 180kDa; Ribosome binding protein 1 homolog; Ribosome receptor protein; RRBP 1; RRp; RRBP |
| 研究领域 | 肿瘤 细胞生物 免疫学 肿瘤细胞生物标志物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 152 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RRBP1: 641-740/1410 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
RRBP1 (Ribosome-binding protein 1) acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Function: Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane (By similarity). Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. SWISS: Q9P2E9 Gene ID: 6238 Database links: Entrez Gene: 6238 Human Entrez Gene: 81910 Mouse Omim: 601418 Human SwissProt: Q9P2E9 Human SwissProt: Q99PL5 Mouse Unigene: 472213 Human Unigene: 13705 Mouse |
| 产品图片 |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RRBP1 antibody (bs-6574R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
