| 产品编号 | bs-6552R |
| 英文名称 | NANOGP8 Rabbit pAb |
| 中文名称 | 干细胞转录因子NANOGP8抗体 |
| 别 名 | Homeobox protein NANOGP8; MGC119250; NANOG; Nanog homeobox pseudogene 8; NANOGP 8; NANOGP1; NANOGP8; NANP8_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Rabbit,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NANOGP8: 101-200/305 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009] Function: May act as a transcription regulator. When overexpressed, promotes entry of cells into S phase and cell proliferation. Subcellular Location: Nucleus. Tissue Specificity: Expressed in osteosarcoma cancer cell line (at protein level). breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines. Similarity: Belongs to the Nanog homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q6NSW7 Gene ID: 388112 Database links: Entrez Gene: 388112 Human SwissProt: Q6NSW7 Human |
| 产品图片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-NANOGP8 (bs-6552R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
