| 产品编号 | bs-6515R |
| 英文名称 | ABC2 Rabbit pAb |
| 中文名称 | 乳腺癌增强蛋白2抗体 |
| 别 名 | ABC 2; ABC2; ABC-2; Amplified in breast cancer gene 2 protein; Chromosome 17 open reading; chromosome 17 open reading frame 71; Protein smg 8 homolog; protein SMG8; SMG8; SMG8 protein; SMG8_HUMAN. |
| 研究领域 | 肿瘤 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 110 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ABC2/C17orf71: 721-820/991 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
C17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.Positive control;Human Anaplastic Thyroid Carcinoma, Human Breast Carcinoma. Function: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG9 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required to mediate the recruitment of SMG1 to the ribosome:SURF complex and to suppress SMG1 kinase activity until the ribosome:SURF complex locates the exon junction complex (EJC). Acts as a regulator of kinase activity. Subunit: Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Forms heterodimers with SMG9; this assembly form may represent a SMG1C intermediate form. Subcellular Location: Phosphorylated by SMG1 Post-translational modifications: Phosphorylated by SMG1. Similarity: Belongs to the SMG8 family. SWISS: Q8ND04 Gene ID: 55181 Database links: Entrez Gene: 55181 Human Omim: 613175 Human SwissProt: Q8ND04 Human |
| 产品图片 |
Sample:
K562 (Human) Cell Lysate at 30 ug
Primary: Anti- ABC2 (bs-6515R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110 kD
Observed band size: 110 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
