产品编号 | bs-6298R |
英文名称 | OVCA2 Rabbit pAb |
中文名称 | 卵巢癌相关基因2蛋白抗体 |
别 名 | Candidate tumor suppressor in ovarian cancer 2; Candidate tumor suppressor OVCA2; ovarian cancer associated gene 2 protein; Ovarian cancer gene 2 protein; ovarian tumor suppressor candidate 2; OVCA2_HUMAN. |
研究领域 | 肿瘤 染色质和核信号 信号转导 转录调节因子 肿瘤细胞生物标志物 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 24 kDa |
检测分子量 | |
细胞定位 | 细胞核 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human OVCA2: 11-100/227 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Ubiquitously expressed protein which belongs to the UPF0483 (OVCA2) family. Encoded in an intron of the gene DPH1/OVCA1 (same strand). Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Proteolytically degraded in response to RA and 4HPR treatment in a time- and dose-dependent manner in the promyelocytic leukemia cell line HL-60. Similarity: Belongs to the UPF0483 (OVCA2) family. SWISS: Q8WZ82 Gene ID: 124641 Database links: Entrez Gene: 124641 Human Entrez Gene: 246257 Mouse Omim: 607896 Human SwissProt: Q8WZ82 Human SwissProt: Q9D7E3 Mouse Unigene: 513856 Human Unigene: 41496 Mouse |
产品图片 |
Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OVCA2 Polyclonal Antibody, Unconjugated(bs-6298R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |