| 产品编号 | bs-6261R |
| 英文名称 | NEK3 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶Nek3抗体 |
| 别 名 | HSPK 36; HSPK36; HSPK-36; NEK3; NEK 3; NEK-3; NEK3_HUMAN; Never in mitosis A-related kinase 3; NIMA(never in mitosis gene a) related kinase 3; NimA related protein kinase 3; NimA-related protein kinase 3; Serine/threonine protein kinase Nek3; Serine/threonine-protein kinase Nek3. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEK3: 165-265/506 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar2009]. Function: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. Subunit: Interacts with PXN, PRLR, VAV1 and VAV2 and this interaction is prolactin-dependent. Subcellular Location: Cytoplasm (By similarity). Cell projection, axon (By similarity). Tissue Specificity: Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain. Post-translational modifications: Phosphorylation at Thr-479 regulates its catalytic activity (By similarity). Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain. SWISS: P51956 Gene ID: 4752 Database links: Entrez Gene: 4752 Human Entrez Gene: 23954 Mouse Omim: 604044 Human SwissProt: P51956 Human SwissProt: Q9R0A5 Mouse Unigene: 409989 Human Unigene: 41413 Mouse Unigene: 45730 Rat |
| 产品图片 |
Sample:
Small intestine (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Urinary bladder (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-NEK3 (bs-6261R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
