产品编号 | bs-6230R |
英文名称 | Rabbit Anti-LC3C antibody |
中文名称 | 微管相关蛋白轻链3C抗体 |
别 名 | LC3 like protein 2; MAP1 light chain 3 like protein 3; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma; MAP1LC3C; MLP3C_HUMAN. |
研究领域 | 心血管 信号转导 细胞骨架 细胞自噬 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Rat,Zebrafish (predicted: Pig,Cow,Chicken,Dog) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 16kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LC3C: 31-126/147 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. There are three isoforms of human LC3 (named MAP1LC3A, MAP1LC3B, and MAP1LC3C), which exhibit different tissue distributions. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Subcellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Tissue Specificity: Most abundant in placenta, lung and ovary. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9BXW4 Gene ID: 440738 Database links: Entrez Gene: 440738 Human Omim: 609605 Human SwissProt: Q9BXW4 Human Unigene: 534971 Human |
产品图片 |
Sample: Heart (Rat) Lysate at 40 ug
Primary: Anti-LC3C (bs-6230R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 16 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3C) Polyclonal Antibody, Unconjugated (bs-6230R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |