| 产品编号 | bs-6211R |
| 英文名称 | PCMT1 Rabbit pAb |
| 中文名称 | 天门冬氨酸甲基转移酶PCMT抗体 |
| 别 名 | L isoaspartyl protein carboxyl methyltransferase; L isoaspartyl/D aspartyl methyltransferase; PCMT 1; PIMT; Protein beta aspartate methyltransferase; Protein L isoaspartate(D aspartate) O methyltransferase; PIMT_HUMAN; Protein L isoaspartyl/D aspartyl methyltransferase. |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PCMT1: 165-227/227 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PCMT1 (Protein-L-isoaspartate (D-aspartate) O-methyltransferase) plays a role in the repair and/or degradation of damaged proteins by initiating the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. It catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues. Function: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity). Subunit: Monomer. Subcellular Location: Cytoplasm. Similarity: Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family. SWISS: P22061 Gene ID: 5110 Database links: Entrez Gene: 5110 Human Omim: 176851 Human SwissProt: P22061 Human SwissProt: P23506 Mouse Unigene: 279257 Human Unigene: 258431 Mouse Unigene: 86985 Rat |
| 产品图片 |
Sample:
Hippocampus (Mouse) Lysate at 40 ug
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- PCMT1 (bs-6211R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCMT1) Polyclonal Antibody, Unconjugated (bs-6211R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
