| 产品编号 | bs-6191R |
| 英文名称 | H3-K4-HMTase SETD7 Rabbit pAb |
| 中文名称 | 组蛋白H4-K4甲基转移酶抗体 |
| 别 名 | FLJ21193; H3 K4 HMTase; H3-K4-HMTase SETD7; H4 lysine 4 specific; Histone H3 K4 methyltransferase; Histone H3 lysine 4 specific methyltransferase; Histone H3-K4 methyltransferase SETD7; Histone H4 K4 methyltransferase; Histone lysine N methyltransferase; Histone lysine N methyltransferase H3 lysine 4 specific SET7; Histone-lysine N-methyltransferase SETD7; KIAA1717; KMT7; Lysine methyltransferase; Lysine N-methyltransferase 7; OTTHUMP00000164543; OTTHUMP00000220049; SET 7; SET 7/9; SET 9; SET D7; SET domain containing(lysine methyltransferase) 7; SET domain containing protein 7; SET domain containing protein 8; SET domain-containing protein 7; SET7; SET7/9; SET9; Setd7; SETD7_HUMAN. |
| 研究领域 | 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SETD7: 271-366/366 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro. Function: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Subunit: Interacts with IPF1/PDX-1. Subcellular Location: Nucleus. Chromosome (Probable). Tissue Specificity: Widely expressed. Expressed in pancreatic islets. Similarity: Belongs to the histone-lysine methyltransferase family. SET7 subfamily. Contains 3 MORN repeats. Contains 1 SET domain. SWISS: Q8WTS6 Gene ID: 80854 Database links: Entrez Gene: 80854 Human Entrez Gene: 73251 Mouse Omim: 606594 Human SwissProt: Q8WTS6 Human SwissProt: Q8VHL1 Mouse Unigene: 480792 Human Unigene: 192111 Mouse |
| 产品图片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: U87MG (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-H3-K4-HMTase SETD7 (bs-6191R) IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 47 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
