| 产品编号 | bs-6150R |
| 英文名称 | GPR124 |
| 中文名称 | G蛋白偶联受体124抗体 |
| 别 名 | TEM5; DKFZp434C211; DKFZp434J0911; FLJ14390; G protein coupled receptor 124; G-protein coupled receptor 124; GP124_HUMAN; GPR 124; GPR124; KIAA1531; TEM 5; TEM5; Tumor endothelial marker 5; Tumor endothelial marker 5 precursor. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 血管内皮细胞 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 139kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR124: 1201-1303/1303 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Tumor endothelial markers (TEMs) display elevated expression during angiogenesis. Their associations with the cell surface membrane are of great interest for both biological and clinical research. G protein coupled receptor 124 (GPR124) is encoded as a seven pass transmembrane protein of 1331 amino acids and is most similar to the cadherin related Celsr1 proteins and members of the calcium independent alpha latrotoxin receptor family involved in transmitting signals across the cell membrane. GPCRs have been excellent drug targets because their natural ligands can often mimicked for agonistic or antagonistic purpose. The existence of tumour specific endothelial markers provides a resource for further understanding the mechanisms of tumour angiogenesis and encourages the development of antiangiogenic therapies in the future. ESTs for the gene that encodes GPR124 have been isolated primarily from amnion, connective tissue, ear, embryo, eye, ganglion, heart, lung, placenta, and skin libraries. Function: Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). Subunit: Interacts with DLG1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Similarity: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 1 GPS domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 4 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q96PE1 Gene ID: 25960 Database links: Entrez Gene: 25960 Human Entrez Gene: 78560 Mouse Omim: 606823 Human SwissProt: Q96PE1 Human SwissProt: Q91ZV8 Mouse Unigene: 274136 Human Unigene: 87046 Mouse |
| 产品图片 |
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Large intestine (Rat) Lysate at 40 ug Lane 4: Lung (Rat) Lysate at 40 ug Lane 5: U2os (Human) Cell Lysate at 30 ug Primary: Anti-GPR124 (bs-6150R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 180-200 kD Observed band size: 245 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
