| 产品编号 | bs-6138R |
| 英文名称 | EID1 Rabbit pAb |
| 中文名称 | 乙酰基转移酶EID1抗体 |
| 别 名 | 21 kDa pRb associated protein; C15orf3; CBP; Chromosome 15 open reading frame 3; CREBBP/EP300 inhibitor 1; CREBBP/EP300 inhibitory protein 1; CRI 1; CRI1; E1A like inhibitor of differentiation; EID 1; EP300 interacting inhibitor of differentiation 1; NB4 apoptosis related protein; PNAS 22; PNAS22; PTD014; Rb and p300 binding protein EID 1; Rb and p300 binding protein EID1; RBP21; Retinoblastoma protein associated protein; EID1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 信号转导 干细胞 细胞凋亡 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EID1: 111-187/187 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
EID1 ia an acetyltransferase enzyme. It acetylates histones, giving a specific tag for transcriptional activation. EID1 also acetylates non histone proteins, like NCOA3 coactivator. It Mediates cAMP gene regulation by binding specifically to phosphorylated CREB protein and as coactivator, augments the activity of phosphorylated CREB to activate transcription of cAMP responsive genes. Function: Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. Subunit: Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27. Subcellular Location: Nucleus. Cytoplasm. Note=May shuttle between nucleus and cytoplasm. Tissue Specificity: Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A-549 and various leukemia cell lines. Post-translational modifications: Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. SWISS: Q9Y6B2 Gene ID: 23741 Database links: Entrez Gene: 23741 Human Entrez Gene: 58521 Mouse Omim: 605894 Human SwissProt: Q9Y6B2 Human SwissProt: Q9DCR4 Mouse Unigene: 255973 Human Unigene: 425101 Mouse Unigene: 472964 Mouse Unigene: 22737 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
