产品编号 | bs-6103R |
英文名称 | SEZ6L Rabbit pAb |
中文名称 | 癫痫样相关蛋白6抗体 |
别 名 | Seizure 6 like protein; Seizure related 6 homolog(mouse) like; Seizure related gene 6(mouse) like; Seizure related gene 6 like; SE6L1_HUMAN. |
研究领域 | 免疫学 神经生物学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Chicken,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 109 kDa |
检测分子量 | |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SEZ6L: 701-800/1024 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
SEZ6L is a candidate tumor suppressor gene. This protein may contribute to specialized endoplasmic reticulum functions in neurons. Function: May contribute to specialized endoplasmic reticulum functions in neurons Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein Tissue Specificity: Widely expressed, including adult and fetal brains and lungs. Not expressed in all lung cancer cell lines. Similarity: Belongs to the SEZ6 family. Contains 3 CUB domains. Contains 5 Sushi (CCP/SCR) domains. SWISS: Q9BYH1 Gene ID: 23544 Database links: Entrez Gene: 23544 Human Entrez Gene: 56747 Mouse Omim: 607021 Human SwissProt: Q9BYH1 Human SwissProt: Q6P1D5 Mouse Unigene: 194766 Human Unigene: 143742 Mouse Unigene: 209285 Rat |
产品图片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- SEZ6L (bs-6103R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 109 kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEZ6L) Polyclonal Antibody, Unconjugated (bs-6103R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |