| 产品编号 | bs-6100R |
| 英文名称 | SESN3 Rabbit pAb |
| 中文名称 | Sestrin3抗体 |
| 别 名 | SEST3; 5630400E15Rik; SESN3_HUMAN; SESN3; 1.11.1.-; SESN3_MOUSE; |
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Specific References (2) | bs-6100R has been referenced in 2 publications.
[IF=5.168] Kosaka et al. Reactive oxygen species induction by cabazitaxel through inhibiting Sestrin-3 in castration resistant prostate cancer. (2017) Oncotarget. 8:87675-87683 IF(ICC) ; Human.
[IF=5.14] Agata Wrońska. et al. Sestrins’ Expression in the Liver Is Not Altered by Short-Term Calorie Restriction in Young and Old Rats. Gerontology. ;:1-9 WB ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,GuineaPig,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SESN3: 401-492/492 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Subcellular Location: Widely expressed. Similarity: Belongs to the sestrin family. SWISS: P58005 Gene ID: 143686 Database links: Entrez Gene: 143686 Human Entrez Gene: 75747 Mouse Omim: 607768 Human SwissProt: P58005 Human SwissProt: Q9CYP7 Mouse Unigene: 659934 Human Unigene: 325126 Mouse Unigene: 439776 Mouse |
| 产品图片 |
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- SESN3 (bs-6100R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 52 kD
Sample:
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- SESN3 (bs-6100R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 56 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
