| 产品编号 | bs-6095R |
| 英文名称 | RND3 Rabbit pAb |
| 中文名称 | Rho家族GTP酶3抗体 |
| 别 名 | ARHE; memB; Rho family GTPase 3; Rho8; RhoE; RND3_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 染色质和核信号 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 27 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RND3: 51-150/244 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Rho family of proteins mediates cytoskeleton organization and cell motility responses. This family contains twenty different members divided into five groups, the Rho-like, Rac-like, Cdc42-like, Rnd and RhoBTB subfamilies. Rho proteins have GTPase activity and bind GTP in their active form and GDP in their inactive form. In their active form they interact with downstream proteins. However, the Rnd subfamily of proteins containing the Rnd1, Rnd2 and RhoE(Rnd3), binds GTP but not GDP and has no GTPase activity. While Rho proteins are required for stress fibre formation in cultured fibroblasts, epithelial and endothelial cells, overexpression of Rnd3 or Rnd1 in the same cells may cause an opposite effect (i.e. decrease in stress fibre). Thus, the Rnd3 protein may act as an antagonist for the Rho proteins either by directly binding to Rho or to other downstream proteins. Subunit: Binds ROCK1 (By similarity). Interacts with UBXD5. Subcellular Location: Golgi Apparatus; Golgi apparatus membrane; peripheral membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the small GTPase superfamily. Rho family. SWISS: P61587 Gene ID: 390 Database links: Entrez Gene: 390 Human Entrez Gene: 74194 Mouse Omim: 602924 Human SwissProt: P61587 Human SwissProt: P61588 Mouse Unigene: 6838 Human Unigene: 46497 Mouse Unigene: 25153 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RND3) Polyclonal Antibody, Unconjugated (bs-6095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RND3) Polyclonal Antibody, Unconjugated (bs-6095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
