| 产品编号 | bs-6033R |
| 英文名称 | Rabbit Anti-Contactin 3 antibody |
| 中文名称 | 脑源性免疫球蛋白超家族蛋白3抗体 |
| 别 名 | BIG 1; BIG-1; Contactin-3; Plasmacytoma-associated neuronal glycoprotein; Brain derived immunoglobulin superfamily protein 1; Brain-derived immunoglobulin superfamily protein 1; KIAA1496; PANG; PCS; CNTN3. |
| 研究领域 | 细胞生物 免疫学 神经生物学 细胞表面分子 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 108kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Contactin 3: 301-400/1028 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Contactins mediate cell surface interactions during nervous system development. Contactin 3 has some neurite outgrowth-promoting activity. Function: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity (By similarity). Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: In brain, it is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Similarity: Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q9P232 Gene ID: 5067 Database links: Entrez Gene: 5067 Human Entrez Gene: 18488 Mouse Omim: 601325 Human SwissProt: Q9P232 Human SwissProt: Q07409 Mouse Unigene: 12723 Human Unigene: 435479 Mouse Unigene: 10043 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Contactin 3 Polyclonal Antibody, Unconjugated(bs-6033R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
