| 产品编号 | bs-2932R |
| 英文名称 | Clathrin heavy chain Rabbit pAb |
| 中文名称 | 网格蛋白重链抗体 |
| 别 名 | CHC17; Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain; Clathrin heavy chain like 1; Clathrin heavy chain on chromosome 17; Clathrin heavy polypeptide; CLH 17; CLH 22; CLH-17; CLH1_HUMAN; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034. |
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Specific References (1) | bs-2932R has been referenced in 1 publications.
[IF=0] Mörke, Caroline. ""Caveolae-mediated phagocytosis of biomaterial surface structures by human osteoblasts."Universität Rostock, 2016. IF(ICC) ; Human.
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| 研究领域 | 细胞生物 免疫学 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Bee,Ant,Frog,Silkworm) |
| 产品应用 | WB=1:500-2000,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 192 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Clathrin heavy chain.: 401-500/1675 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]. Function: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Subunit: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1. Interacts with DENND1A, DENND1B and DENND1C (By similarity). May interact with OCRL. Interacts with ERBB2 (By similarity). Subcellular Location: Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the clathrin heavy chain family. Contains 7 CHCR (clathrin heavy-chain) repeats. SWISS: Q00610 Gene ID: 1213 Database links: Entrez Gene: 1213 Human Entrez Gene: 67300 Mouse Omim: 118955 Human SwissProt: Q00610 Human SwissProt: Q68FD5 Mouse Unigene: 491351 Human Unigene: 479526 Mouse Unigene: 3589 Rat |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Clathrin heavy chain polyclonal antibody, unconjugated (bs-2932R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Clathrin heavy chain) polyclonal Antibody, Unconjugated (bs-2932R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
