| 产品编号 | bs-6006R |
| 英文名称 | FBXO31 Rabbit pAb |
| 中文名称 | F-box蛋白家族FBXO31抗体 |
| 别 名 | FBX14; FBXO14; Fbx31; MRT45; pp2386; 1110003O08Rik; 2310046N15Rik; FBX31_HUMAN; FBXO31; FBX31_MOUSE; |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FBXO31: 151-250/539 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
FBXO31 belongs to the F-box protein family. Such proteins are characterized by an F-box motif of approximately 40 residues. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains. There are two different isoforms. Function: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. Subunit: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Tissue Specificity: Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. Post-translational modifications: Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization. Similarity: Belongs to the FBXO31 family. Contains 1 F-box domain. SWISS: Q5XUX0 Gene ID: 79791 Database links: Entrez Gene: 79791 Human Entrez Gene: 76454 Mouse Omim: 609102 Human SwissProt: Q5XUX0 Human SwissProt: Q3TQF0 Mouse Unigene: 567582 Human Unigene: 467749 Mouse |
| 产品图片 |
Sample: NIH/3T3 cell (mouse) Lysate at 40 ug
Primary: Anti-FBXO31 (bs-6006R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO31) Polyclonal Antibody, Unconjugated (bs-6006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO31) Polyclonal Antibody, Unconjugated (bs-6006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
