| 产品编号 | bs-5984R |
| 英文名称 | ABI2 Rabbit pAb |
| 中文名称 | 肿瘤抑制基因ABI2/精氨酸酪氨酸蛋白激酶结合蛋白抗体 |
| 别 名 | Abelson interactor 2; ABI 2; ABI2B; Abl binding protein 3; Abl interacting protein 1(SH3 containing protein); Abl interactor 2; Abl interactor protein 2b; AblBP3; AIP 1; Arg binding protein 1; Arg protein tyrosine kinase binding protein; ArgBP1; ARGBPIA; ArgBPIB; SSH3BP2; ABI2_HUMAN. |
| 研究领域 | 肿瘤 免疫学 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ABI2: 421-513/513 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ABI2 (Abelson interactor 2) belongs to the ABI family. The Abelson interactors are among a group of proteins that interact with Abl tyrosine kinase, a non-receptor tyrosine kinase whose activation results in the regulation of cytoskeleton function, cell growth and apoptosis in response to a variety of biological stimuli.
Function: May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA. [SUBUNIT] Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Interacts with ABL1 and ABL2. Subcellular Location: Cytoplasm (By similarity). Isoform 1: Cell projection, lamellipodium (By similarity). Cell projection, filopodium (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips (By similarity). Post-translational modifications: Is a substrate for ABL1. Similarity: Belongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain. SWISS: Q9NYB9 Gene ID: 10152 Database links: Entrez Gene: 10152 Human Entrez Gene: 329165 Mouse Omim: 606442 Human SwissProt: Q9NYB9 Human SwissProt: P62484 Mouse Unigene: 471156 Human Unigene: 212066 Mouse Unigene: 465571 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABI2) Polyclonal Antibody, Unconjugated (bs-5984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABI2) Polyclonal Antibody, Unconjugated (bs-5984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
