| 产品编号 | bs-4292R |
| 英文名称 | BPI Rabbit pAb |
| 中文名称 | 杀菌/通透性增强蛋白抗体 |
| 别 名 | Neutrophil Bactericidal Protein BP30; Bactericidal permeability-increasing protein; Bactericidal/permeability increasing protein; BPI_HUMAN; CAP 57; CAP-57; BPI fold containing family D, member 1; BPIFD1. |
| 研究领域 | 细胞生物 免疫学 细胞凋亡 细菌及病毒 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BPI: 141-240/487 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. Function: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Subunit: Monomer. Homodimer; disulfide-linked. Subcellular Location: Secreted. Cytoplasmic granule membrane. Note=Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. Tissue Specificity: Restricted to cells of the myeloid series. Similarity: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. SWISS: P17213 Gene ID: 671 Database links: Entrez Gene: 671 Human Omim: 109195 Human SwissProt: P17213 Human Unigene: 529019 Human 杀菌／通透性增加蛋白(Bactericidal／permeability-increasing protein，BPI)是哺乳动物中性粒细胞中存在的一种碱性蛋白，BPI能与革兰氏阴性菌脂多糖结合，增加外膜对抗菌药物的通透性，具有中和内毒素和杀灭细菌的生物学作用，在革兰氏阴性菌感染的治疗方面有良好的发展前景。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
