[IF=14.903] Gaona Shi. et al. Bone-derived MSCs encapsulated in alginate hydrogel prevent collagen-induced arthritis in mice through the activation of adenosine A2A/2B receptors in tolerogenic dendritic cells. ACTA PHARM SIN B. 2023 Apr;:  WB ;  Mouse.  

PubMed:10.1016/j.apsb.2023.04.003

[IF=4.848] Alina V. Meyer. et al. Host CD39 Deficiency Affects Radiation-Induced Tumor Growth Delay and Aggravates Radiation-Induced Normal Tissue Toxicity. Front Oncol. 2020; 10: 554883  FC ;  Mouse.  

PubMed:33194619

[IF=3.061] Shi L et al. Adenosine Interaction with Adenosine Receptor A2a Promotes Gastric Cancer Metastasis by Enhancing PI3K–AKT–mTOR Signaling. Mol Biol Cell. 2019 Jul 24:mbcE19030136.  WB,IHC&ICF ;  Human.  

PubMed:31339445

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-ADORA2B (bs-5900R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 37 kD
Observed band size: 36 kD

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Lane 5: A431 (Human) cell Lysate at 30 ug
Lane 6: U251 (Human) cell Lysate at 30 ug
Primary: Anti-ADORA2B (bs-5900R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34-41 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (bs-5900R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (bs-5900R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: A549.
Primary Antibody (green line): Rabbit Anti-ADORA2B antibody (bs-5900R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.