| 产品编号 | bs-5889R |
| 英文名称 | Rabbit Anti-Hyaluronidase3 antibody |
| 中文名称 | 透明质酸酶3/玻璃酸酶3抗体 |
| 别 名 | Hyaluronidase 3; HYAL3; Hyaluronidase-3; Hyaluronoglucosaminidase 3; LUCA14; LUCA3; Minna14; HYAL3_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 47kDa |
| 细胞定位 | 细胞浆 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hyaluronidase3: 51-150/417 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. Subcellular Location: Secreted (By similarity). Lysosome (By similarity). Tissue Specificity: Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. Similarity: Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. SWISS: O43820 Gene ID: 8372 Database links: Entrez Gene: 8372 Human Entrez Gene: 109685 Mouse Omim: 604038 Human SwissProt: O43820 Human SwissProt: Q8VEI3 Mouse Unigene: 129910 Human Unigene: 729310 Human Unigene: 447916 Mouse Unigene: 72927 Rat |
| 产品图片 |
Sample:
Mouse(Kidney) Lysate at 40 ug
Primary: Anti- Hyaluronidase3 (bs-5889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Stomach tissue lysates
Primary: Anti-Hyaluronidase3 (bs-5889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 53 kDa
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody (bs-5889R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
