| 产品编号 | bs-5876R |
| 英文名称 | MMP-28 Rabbit pAb |
| 中文名称 | 基质金属蛋白酶28抗体 |
| 别 名 | Epilysin; Matrix Metalloproteinase 28; MM 28; MM28; MMP 28; MMP-28; MMP28. |
| 研究领域 | 肿瘤 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Dog,GuineaPig) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP28: 201-300/520 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Matrix Metalloproteinase 28, also known as epilysin, was first cloned from human skin keratinocytes and described to reflect its role in the remodeling of the epidermis. It was found in testis, as well as heart, brain, placenta, lung, prostate, intestine, and colon. MMP28 was later cloned from human lung and found in lung, kidney, brain, skeletal muscle, and several tumor cell lines. At least three MMP28 transcripts of 2.6, 2.0, and 1.2 kb have been reported possibly representing alternatively spliced forms of MMP28. There are two human sequences (isoform1 and isoform 2) which encode proteins of 520 and 393 amino acids with predicted masses of 58.9 and 44.5 kDa respectively. The mouse sequence contains two inserts of 41 and 39 amino acids respectively, relative to the human sequence. Mouse epilysin has a predicted molecular mass of approx. 70 kDa. Mouse and human epilysin are highly conserved and share 97% identical residues. Epilysin (MMP-28) contains the key domains of the other MMPs: a signal peptide, conserved cysteine-containing prodomain (with a furin cleavage site), conserved histidine-containing catalytic domain, hinge domain, and hemopexin domain. MMP28 has a furin cleavage site, similar to MMP11, and is cleaved by the prohormone convertase family of enzymes. Sequence identity between MMP28 and the other MMPs is low overall. MMP28 is most closely related to MMP19 (39% sequence identity). Recombinant MMP28 degrades casein. Function: Can degrade casein. Could play a role in tissues homeostasis and repair. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytesis. Widely expressed in several carcinomas as well. Is up-regulated in response to injury in the skin. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: Q9H239 Gene ID: 79148 Database links: Entrez Gene: 79148 Human Entrez Gene: 118453 Mouse Omim: 608417 Human SwissProt: Q9H239 Human Unigene: 380710 Human Unigene: 264648 Mouse Unigene: 60472 Rat |
| 产品图片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Ovary tissue lysates
Lane 3: Mouse Raw264.7 cell lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Kidney tissue lysates
Lane 6: Human MCF-7 cell lysates
Lane 7: Human HeLa cell lysates
Primary: Anti-MMP-28 (bs-5876R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
